Welcome on ANR ISEAPIM

​Acylated amino acids or peptides are increasingly used, mainly as biosurfactants in ecologically friendly cleaning products, cosmetic and pharmaceuticals. Within the framework of sustainable development, the aim of this project is therefore to design and intensify alternative and sustainable continuous acylation processes (with controlled regio/chemo selectivity) by using (i) enzymatic heterogeneous catalysis (ii) innovative carriers for enzymes immobilization/stabilization, and (iii) eco-compatible/recyclable solvents. 

Considering both enzymes and reaction media, the ISEAPIM3 project will be structured around two main axes.
The first research axis will investigate the use of supercritical carbon dioxide (scCO2) as solvent and the well-known commercial lipase B from Candida antarctica (CAL B).
The second axis will investigate the use of oil in water emulsion as solvent and one or several amino-acylases discovered from Streptomyces ambofaciens.
In order to avoid enzymes leaching in continuous reactors, the use of covalent binding and cross linked enzymes aggregates as immobilization/stabilization technics will be performed by the use of promising macro/mesoporous siliceous materials (monoliths and beads) for enzymes immobilization (SRSMC, CRPP).
Given the numerous factors involved in the continuous process (temperature, pressure, flow, density, enzyme loading, substrates ratios, carrier porosity, reactor diameter and length…) design of experiments (DOE) combined with a structured process intensification analysis fed with collected experimental data will be used to reduce the number of requested experiments and achieve a mechanistic knowledge on limiting factors as well as on levers on which we should play for process optimization (​LRGP).
The use of high pressure CO2 (300-1000 bars) for amino acid acylation will be also explored. Synthetized products, some of which will be of direct interest for the industrial partner (​SEPPIC) of the present project, will be subjected to techno-functional and biological tests.

Finally, the knowledge achieved during the project will be used for the establishment of a Life cycle Assessment in order to evaluate whether our enzymatic acylation processes can effectively compete with the Schotten-Baumann chemical acylation. 

Scientific coordinator

Tél: +33 (0) 3 83 17 52 21
1 rue Grandville
BP 20451
54001 NANCY cedex, France


Fabrice BLANCHARD, LRGP, Nancy, France
Isabelle CHEVALOT, LRGP, Nancy, France
Stéphane DELAUNAY, LRGP, Nancy, France
Xavier FRAMBOISIER, LRGP, Nancy, France
Frantz FOURNIER, LRGP, Nancy, France
Eric OLMOS, LRGP, Nancy, France
Danielle BARTH, LRGP, Nancy, France
Jean-Marc COMMENGE, LRGP, Nancy, France
Hervé SIMONAIRE, LRGP, Nancy, France
Léna DETTORI, LRGP, Nancy, France

Jean-Luc BLIN, SRSMC, Nancy, France
Mélanie EMO, SRSMC, Nancy, France
Marie-Joe STEBE, SRSMC, Nancy, France
François VIVERT, SRSMC, Nancy, France
​Chafik BOURKAIB, SRSMC, Nancy, France

Rénal BACKOV, CRPP, Bordeaux, France
Eric LAURICHESSE, CRPP, Bordeaux, France             
Armand ROUCHER, CRPP, Bordeaux, France  
Véronique SCHMITT, CRPP, Bordeaux, France   

Sandy DUMONT, 
SEPPIC, Puteaux, France
Jérome GUILBOT, SEPPIC, Puteaux, France
François GUY, SEPPIC, Puteaux, France            
Cécile TAILLEBOIS, SEPPIC, Puteaux, France