The aim of the present project is to design and intensify sustainable enzymatic acylation processes of amino acids or peptides without need for using the Schotten-Baumann chemical acylation reaction.
To do so, the strategy will concern the use of:

- Appropriate enzymatic catalysts
- Siliceous macroporous and meso-macroporous monoliths (but also beads) on which enzymes will be immobilized
- Eco-compatible and enzyme-compatible reaction media (CO2 and water)

In this context, and as simply shown in Figure 1., a particular attention will be paid on enzymatic continuous acylation processes by systematically keeping in mind the relevance of the association “reaction media/enzyme/support for enzyme immobilization” with a double objective of continuous process description/intensification & control of reaction selectivity in terms of regio-, chemo of acylation.

Scientific Program

Studies will be focused on two following main research axes (Figure 2.)

  • The first axis will investigate the use of supercritical carbon dioxide (scCO2) as an eco-compatible solvent for amino acids or peptides acylation by lipase immobilized within innovative siliceous macroporous materials (monoliths or beads), acrylic macroporous beads or via the use of cross linked enzymes aggregates (CLEAs). The lipase considered in this first axis will be the commercial lipase B from Candida antarctica.
  • The second axis will investigate the use of oil in water emulsion as an ecocompatible reaction medium and immobilized acylases with similar carriers for acylase immobilization.

The global architecture of the project is depicted in the following figure :